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Optimization Through AI Vision

AI Vision for the Operating Room & Cell & Gene Therapy

Computer vision and AI that streamline surgical workflows, eliminate instrument redundancy, and automate CGT manufacturing.

AI-powered surgical vision system

60%

Reduced Instrument Redundancy

Decreased Contamination Risk

8 min

Turnover Time Saved Per Case

>6×

First-Year ROI

Trusted Academic & Research Partners

The Problem

Operational Inefficiencies at Scale

Hospitals and CGT manufacturers face mounting cost pressures from labor-intensive workflows.

Hospital Operating Rooms

Bloated trays, misplaced instruments, and poor data sharing between OR and SPD inflate costs and delay procedures.

$3
Cost per instrument
$60
OR cost per minute
$20M
Annual waste

CGT Manufacturing

Manual processes and bioreactor constraints remain cost prohibitive, limiting patient access.

$1M
Cost per patient
1012 vg/kg
Suspension cell yield

SPD-OR Platform

Intelligent Surgical Workflow

AI cameras that transform instrument tracking, tray composition, and OR turnover.

AI-identified surgical instruments with color-coded tracking

Real-Time Instrument Identification

Our AI classifies every instrument in the tray, tracks utilization, flags redundancies, and recommends optimized sets.

At TJUH, we reduced a TLIF set from 100 to 40 instruments — 60% reduction, zero compromise in surgical capability.

60% Tray Reduction 2.7 min / tray saved
📹

Instrument Tracking

Real-time visibility on every instrument throughout each procedure.

📊

Utilization Capture

Identify instruments that rarely leave the tray. Evidence-based optimization.

Workflow Optimization

Each additional tray adds 3 min to turnover. Streamlined sets cut idle time and maximize OR throughput.

🔄

SPD Quality Control

Instrument verification, bioburden detection, and case data for SPD operators.

🏷️

RFID Integration

RFID tagging plus computer vision: a closed-loop ecosystem for 100% visibility.

💰

Value Adoption

At $5,000/surgeon/month, the platform delivers $400K in annual savings.

Bodhi

Automating CGT Production

Suspension cell techniques cost $1M per patient. Bodhi's AI-guided automation dramatically reduces costs and scales access to therapies.

🔬

AI-Guided Microscopy

Automated cell culture inspection monitors development in real time, reducing manual intervention.

🤖

Robotic Process Control

Precision multi-step manufacturing via our automated multi-layered 2D concentric roller bottle platform.

🧬

Scalable AAV Production

HEK-293-based production with fit-to-spec vector design, serotype selection, and optimized yields.

Bodhi AI-driven automated roller bottle system

Bodhi

An automated roller bottle system for adherent AAV production. Real-time monitoring, automated feeding, and environmental control in a compact form factor.

Scale-up that aligns research with commerical manufacturing.

Self-contained platform available in 3 x 3 and 5 x 5 profiles.

AAV production roadmap — from cell expansion to fill and finish

End-to-End AAV Manufacturing

Our fully integrated pipeline covers every stage of AAV production.

🧬 Cell Expansion — HEK-293/HeLa scale-up in Bodhi roller bottles
🔬 Transfection & AAV Generation — Plasmid delivery with real-time monitoring
Purification & QC — Cell lysis, purification, and quality testing
📦 Fill & Finish — GMP-ready packaging for clinical delivery

Why Bodhi Wins

We preserve adherent biology, add automation, and scale out — without the drawbacks of bioreactors or microcarrier systems.

Adherent 2D Platform

Mirrors discovery SOP. Multi-layer concentric bottles preserve process fidelity at scale.

Clear Optics & In-Process QC

Full visual access for AI monitoring. High full/empty capsid ratio. Segmented bottles isolate failures.

Improved Reproducibility

Low early-phase capex. Reproducible yields with high batch comparability.

Programmable Microenvironments

Tailored conditions per bottle to optimize growth across runs.

Research Partnerships

Babraham Research Campus & Beyond

Based at the Babraham Research Campus, Cambridge, UK. Collaborating with leading academic centres to bring AI innovation to clinical practice.

🏥

Thomas Jefferson University

Clinical validation: turnover metrics, instrument burden analysis, and tray optimization across neurosurgical and ENT specialties.

🎓

Temple University

NIH SBIR partnership: Automated 2D Cell & Gene Therapy Manufacturing Platform.

Publications & Research

Peer-Reviewed Work

Selected publications from our team spanning AI-driven surgical analytics, gene therapy, and neural regeneration.

2026
Artificial Intelligence–Powered Analysis of Operating Room Turnover: Impact of Instrument Burden
Smit RD, Mahtabfar A, Swanepoel E, Evans JJ, Harrop JS
Journal of Neurosurgery, Feb 2026
AI Vision OR Efficiency
JNSPG →
2025
Incorporating AI-Driven Vision Systems to Quantify Learning Curve in EVD Placement
Smit RD, Mahtabfar A, Mouchtouris N, Hines K, Swanepoel E, Bray DP, Evans JJ
Journal of Neurological Surgery Part B: Skull Base, 2025
AI Education Neurosurgery
Thieme →
2024
STAT3 Protects Dopaminergic Neurons Against Degeneration in Animal Model of Parkinson's Disease
Smit RD, Ghosh B, Campion III TJ, Stingel R, Lavell E, Hooper R, Fan X, Soboloff J, Smith GM
Brain Research 1824, 148691
Gene Therapy Neuroprotection
ScienceDirect →
2023
Letter to the Editor: How Should Neurosurgery Approach the Ethical and Legal Considerations for Recording in the Operating Room?
Smit RD, Mahtabfar A, Evans JJ
Journal of Neurosurgery, 140(2):606
Ethics OR Recording
JNSPG →
2022
Viral Expression of Constitutively Active AKT3 Induces CST Axonal Sprouting and Regeneration, but Also Promotes Seizures
Campion III TJ, Sheikh IS, Smit RD, Iffland II PH, Chen J, Bhatt JM, Junker I, Krynska B, Crino PB, Smith GM
Experimental Neurology 349, 113961
Regeneration Viral Vectors
ScienceDirect →
2021
Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector Using Combined Brain and Spine Surgery
Smit RD, Campion III TJ, Stingel RL, Shah PH, Chen J, Smith GM
Journal of Visualized Experiments (JoVE), e62698
Viral Vectors Spinal Cord
JoVE →

Full publication list (26+ papers) available on ResearchGate.

In the News

In the News

The latest from the world of surgical AI, gene therapy, and regenerative medicine.

Appraiseye Press

Team

Experts in Medicine, AI, & CGT

Neurosurgery, AI, gene therapy, and commercialization under one roof.

Rupert Smit

Rupert Smit, MD

Chief Executive Officer

Emil Swanepoel

Emil Swanepoel

Chief Technology Officer

Alwyn Smit

Alwyn Smit

Chief Operating Officer

Carol Ke

Carol Ke, PhD

Chief Scientific Officer

Aria Mahtabfar

Aria Mahtabfar, MD

Director Clinical Strategy

Morgan Schilling

Morgan Schilling

Director Medical Sales

Alexandria Bilder

Alexandria Bilder

Advisor

Contact

Let's Talk

Hospital administrators, CGT manufacturers, academic partners — we'd like to hear from you.

info@appraiseye.com
🌐 appraiseye.io
📍 Philadelphia, PA, USA
📍 Babraham Research Campus, Cambridge, UK